Fall has brought a resurgence of highly pathogenic avian influenza (HPAI) nationwide, and this week the Kansas Department of Agriculture identified HPAI in two Kansas counties: Johnson County and Neosho County. Both new HPAI locations are in non-commercial mixed species flocks, and KDA is working closely with USDA–APHIS on a joint incident response. KDA officials quarantined the affected premises, and birds on both properties will be depopulated to prevent the spread of the disease. Anyone involved with poultry production from the small backyard chicken owner to the large commercial producer should review their biosecurity activities to assure the health of their birds.